Friday, June 4, 2010

Soil Munchers

Aim: To grow micro-organisms from a soil sample.

Materials:
- sample of topsoil containing organic matter
- Bunsen burner, tripod and heatproof mat
- 4 petri dishes with lids - 40mL milk (approximately)
- 2 x 100mL beakers - crucible
- pipe-clay triangle - gauze mat
- matches - stirring rod
- spatula - marking pencil
- narrow masking tape - incubator at 25 degrees.

Method:
1. Complete an RAS before you start this experiment.
2. Set up the apparatus as shown in Figure 8.31. (Refer to textbook)
3. Place about two spatulas of soil in the crucible and heat it for 15 minutes. Stir occasionally with the stirring rod so that it does not overheat. This will sterilize the soil. Allow it to cool.
4. Pour 20mL of milk into one of the beakers and heat it gently until it begins to boil. This will sterilize the milk. Cool the milk.
5. Label the petri dishes 1-4 and add the mixtures specified below.
Dish 1: one spatula of sterilized soil plus 10 mL of sterilized milk.
Dish 2: one spatula of sterilized soil plus 10mL of unsterilized milk.
Dish 3: one spatula of unsterilized soil plus 10mL of sterilized milk.
Dish 4: one spatula of unsterilized soil plus 10mL of unsterilized milk.
6. Seal the lids of the petri dishes by sticking masking tape around the sides. Do not open them again from this point on.
7. Swirl the petri dishes gently to mix the soil and milk.
8. Incubate the dishes at 25 degrees for 4 days.
9. Do not open the petri dishes when you remove them from the incubator.